1 Transposon mutagenesis in a hyper - invasive clinical isolate of Campylobacter 2 jejuni reveals a number of genes with potential roles in invasion . 3 4
نویسندگان
چکیده
3 4 Muhammad Afzal Javed , Andrew J. Grant , Mary. C. Bagnall , Duncan J. Maskell , 5 Diane G. Newell and Georgina Manning. 6 7 8 l School of Science and Technology, Nottingham Trent University, Nottingham, 9 NG11 8NS, U K 10 Department of Veterinary Medicine, University of Cambridge, CB3 OES, UK. 11 Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey, KT15 12 3NB, UK. 13 14 15 16 Running title: Campylobacter jejuni mutants with reduced invasion. 17 18
منابع مشابه
Random transposon mutagenesis of Campylobacter jejuni.
Genetic studies of Campylobacter jejuni have been limited due to the lack of a transposon mutagenesis method. Here, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon into C. jejuni strain 480. Insertions were random, as demonstrated by Southern blot analysis and insertional junction sequencing. We have demonstrated, for the first time, random in vi...
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